paranoia: what if my vial doesnt have estrogen in it

Like what if the vendor forgot to add estrogen to this specific single vial (while the rest of the batch was fine so other users didnt report anything)

How can i tell ? I used another vial before so i cant use stuff like appearance of breastbuds as proof that it has estrogen. Other things are uncertain. What about the dark line that appears under the penis ? How long would it take to disappear after having your estrogen levels fall to male levels ?