Question regarding data presentation
Hi everyone.
I'm very new on FACS/FACS analysis and would appreciate if anyone could help!
I performed three independent cell sorting using different passages of my cells.
I did my analysis and gating on flowjo, and I'm wondering how I can make a visualization showing the percentage of my "P4" cells between passage 1 and passage 2.
I tried using prism but since it's just a single data, the bar graph is just like this and I don't think I could do any statistical testing to it apart from visualization.
I also searched about median fluorescence intensity, and to my understanding that it could describe expression level. I tried finding it with flowjo using my compensated surface marker. But since it's a single point data, does it have any meaning apart from showing trend? Since again I cant do any statistical tests on it. Or am I doing something wrong with the analysis?
Any help would be highly appreciated!